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Bone Marrow Stromal/Stem Cell-Derived EVs Regulate Osteoblast Activity and Differentiation In Vitro and Promote Bone Regeneration In Vivo

Scientists from Shanghai and Gaungzhou, China have presented evidence that an assay they developed using bone marrow stromal-derived extracellular vesicles (EVs) will become a valuable tool for promoting bone regeneration. In their new work, the scientists isolated bone marrow stromal/stem cell (BMSC)-derived EVs through gradient ultracentrifugation and ultrafiltration, and tested the influence of the EVs on osteogenesis, both in vivo and in vitro. The results indicated that EVs positively regulated osteogenic genes and osteoblastic differentiation, but did not inhibit proliferation in vitro. Furthermore, the scientists constructed an EBV delivery system to stimulate bone formation in Sprague Dawley (SD) rats with calvarial defects. They found that BMSC-derived EVs led to more bone formation in the critical-size calvarial bone defects. Furthermore, the scientists constructed an EV delivery system to stimulate bone formation in Sprague Dawley (SD) rats with calvarial defects. They found that BMSC-derived EVs led to more bone formation in the critical-size calvarial bone defects. Moreover, they found that miR-196a plays an essential role in the regulation of osteoblastic differentiation and the expression of osteogenic genes. This work was published online on February 25, 2016 in Scientific Reports. The open-access article is titled “Bone Marrow Stromal/Stem Cell-Derived Extracellular Vesicles Regulate Osteoblast Activity and Differentiation In Vitro and Promote Bone Regeneration In Vivo.”

[Scientific Reports article]